Antibacterial Activity Test of Srikaya Leaf Ethanol Extract (Annona Squamosa L) Against Staphylococcus Epidermidis with Typical Layer Chromatography Profile
Keywords:
Annona Squamosa L., Antibacterial, Staphylococcus EpidermidisAbstract
Srikaya leaves have long been used in traditional medicine for the treatment of cough, fever, rheumatism, diarrhea, and dysentery, as well as an antibacterial and dysentery. The aim of this study is to determine the phytochemical and antibacterial compounds of Srikaya leaf ethanol extract, as well as its antibacterial against Staphylococcus epidermidis bacteria. They also determined the concentration of Srikaya leaf ethanol extract that was most effective in stopping the growth of Staphylococcus epidermidis bacteria. After the Srikaya leaf extract is extracted through the maceration process, phytochemical screening is carried out before the dilution of the extract, which produces 25%, 50% and 75% ethanol concentrations. Disc diffusion method is used to enhance the antibacterial properties of Srikaya leaves on the media Mueller Hinton Agar (MHA). There are alkaloid compounds, flavonoids, tannins, and saponins, according to the findings of this study. With an optimal barrier zone at a concentration of 75% of 11.26 mm, Srikaya leaf extract shows antibacterial capabilities of Staphylococcus epidermidis. It suggests that Srikaya leaf extract contains alkaloids, flavonoids, tannins, and saponins. Further research is needed to find out the antibacterial properties of Srikaya leaves against bacteria such as Bacillus anthraces, Pseudomonas aeruginosa
References
Apriana, R., Rahmawanty, D. and Fitriana, M. (2017) ‘Formulasi Dan Uji Stabilitas Gel Antijerawat Yang Mengandung Kuersetin Serta Uji Efektivitas Terhadap Staphylococcus epidermidis’, Jurnal Pharmascience, 4(2), pp. 187–201. doi: 10.20527/jps. v4i2.5772.
Aryantini, D., Sari, F. and Wijayanti, C. R. (2020) ‘Kandungan Fenolik dan Flavonoid Total Pada Ekstrak Daun Srikaya (Annona squamosa L.) Terfermentasi’, Farmasains: Jurnal Ilmiah Ilmu Kefarmasian, 7(2), pp. 67– 74. doi: 10.22236/farmasains. v7i2.5635.
Datta, F. U. et al. (2019) ‘Uji aktivitas antimikroba bakteri asam laktat cairan rumen terhadap pertumbuhan Salmonella enteritidis, Bacillus cereus, Escherichia coli dan Staphylococcus aureus menggunakan metode difusi sumur agar’, Prosiding Seminar Nasional VII Fakultas Kedokteran Hewan Universitas Nusa Cendana Swiss Bel-inn Kristal Kupang, pp. 66–85.
Devi, S. and Mulyani, T. (2017) ‘Uji Aktivitas Antibakteri Ekstrak Etanol Daun Pacar Kuku (Lawsonia inermis Linn) pada Bakteri Pseudomonas aeruginosa’, Journal of Current Pharmaceutical Sciences ISSN: 2598-2095, 1(1), pp. 30–35.
Erlina, D. V., Hesturini, R. J. and Nurvita, M. (2018) ‘Perbandingan Aktivitas Antibakteri Ekstrak Daun Srikaya Hijau dan Merah (Annona squamosa L.) terhadap Bakteri Staphylococcus aureus’, Prosiding Seminar Nasional Sains, Teknologi dan Analisis, 1, pp. 129–135.
Fitri, I. and Widiyawati, D. (2017) ‘Efektivitas Antibakteri Ekstrak Herba Meniranm (Phylanthus niruni) Terhadap Pertumbuhan Bakteri Salmonella sp. dan Propionibacterium acnes’, JST (Jurnal Sains dan Teknologi), 6(2), pp. 300–310. doi: 10.23887/jst-undiksha. v6i2.11815.
Fitriyanti, Pratama, A. S. and Astuti, K. I. (2019) ‘Uji Efektivitas Antibakteri Infusa Daun Sirsak Tua (Annona muricata L) Terhadap Propionibacterium acnes’, Jurnal Penelitian dan Kajian Ilmiah, 5(2010), pp. 91–96.
Isramilda, I., Sahreni, S. and Saputra, A. I. (2020) ‘Uji Konsentrasi Daya Hambat Rebusan Daun Srikaya (Annona squamosa L.) Terhadap Pertumbuhan Staphylococcus aureus’, BEST Journal (Biology Education, Sains and Technology), 3(1), pp. 01–08. doi: 10.30743/best. v3i1.2378.
Jannah, R., Husni, M. A. and Nursanty, R. (2017) ‘Inhibition Test of Methanol Extract from Soursop Leaf (Annona muricata Linn.) Against Streptococcus mutans Bacteria’, Jurnal Natural, 17(1), pp. 23–30. doi: 10.24815/jn. v17i1.6823.
Legi, A. P., Edy, H. J. and Abdullah, S. S. (2021) ‘Formulation and Antibacterial Test for Liquid Soap with Ethanol Extract of Soursop Leaves (Annona muricata Linn) Against Staphylococcus Aureus Bacteria’, Pharmacon, 10(3), pp.1058–1065.
Notoatmodjo, S. (2018) Metodologi Penelitian Kesehatan. Jakarta: Rineka Cipta.
Rumanti, R. M. et al. (2020) ‘The Comparison of Antibacterial Activities of Soursop Leaf (Annona muricata L.) and Basil Leaf (Ocimum americanum L.) Ethanolic Extracts on Gel Formulated Against Staphilococcus aureus and Propionibacterium’, Asian Journal of Pharmaceutical Research and Development, 8(4), pp. 1–04.
Sandy, M., Wardani, T. S. and Septiarini, A. D. (2021) ‘Uji Aktivitas Antibakteri Ekstrak, Fraksi N-Heksan, Fraksi Etil Asetat, Fraksi Air Daun Pegagan (Centella asiatica (L.) Urb) terhadap Escherichia coli ATCC 25922’, Media Farmasi Indonesia, 16(2), pp. 1683–1692.
Tyastirin, E. and Hidayati, I. (2017) Statistik Parametrik untuk Penelitian Kesehatan. Surabaya: UIN Sunan Ampel. Available at: https://files.osf.io/v1/resources/deuxv/pr oviders/osfstorage/5b684afe7e433e0015 0608d4?action=download&version=1& direct.
Yuda, P. E. S. K., Cahyaningsih, E. and Winariyanthi, N. L. P. Y. (2017) ‘Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Tanaman Patikan Kebo (Euphorbia hirta L.)’, Jurnal Ilmiah Medicamento, 3(2), pp. 61–70
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Sari, Swandono, Prodyanatasari

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Cigarskruie: Journal of Educational and Islamic Research by Saniya Institute is licensed under Creative Commons Attribution ShareAlike 4.0
It means that:
- Adapted Material means material subject to Copyright and Similar Rights that is derived from or based upon the Licensed Material and in which the Licensed Material is translated, altered, arranged, transformed, or otherwise modified in a manner requiring permission under the Copyright and Similar Rights held by the Licensor. For purposes of this Public License, where the Licensed Material is a musical work, performance, or sound recording, Adapted Material is always produced where the Licensed Material is synched in timed relation with a moving image.
- Adapter's License means the license You apply to Your Copyright and Similar Rights in Your contributions to Adapted Material in accordance with the terms and conditions of this Public License.
- BY-SA Compatible License means a license listed at creativecommons.org/compatiblelicenses , approved by Creative Commons as essentially the equivalent of this Public License.
- Copyright and Similar Rights means copyright and/or similar rights closely related to copyright including, without limitation, performance, broadcast, sound recording, and Sui Generis Database Rights, without regard to how the rights are labeled or categorized. For purposes of this Public License, the rights specified in Section 2(b)(1)-(2) are not Copyright and Similar Rights.
- Effective Technological Measures means those measures that, in the absence of proper authority, may not be circumvented under laws fulfilling obligations under Article 11 of the WIPO Copyright Treaty adopted on December 20, 1996, and/or similar international agreements.
- Exceptions and Limitations means fair use, fair dealing, and/or any other exception or limitation to Copyright and Similar Rights that applies to Your use of the Licensed Material.
- License Elements means the license attributes listed in the name of a Creative Commons Public License. The License Elements of this Public License are Attribution and ShareAlike.
- Licensed Material means the artistic or literary work, database, or other material to which the Licensor applied this Public License.
- Licensed Rights means the rights granted to You subject to the terms and conditions of this Public License, which are limited to all Copyright and Similar Rights that apply to Your use of the Licensed Material and that the Licensor has authority to license.
- Licensor means the individual(s) or entity(ies) granting rights under this Public License.
- Sui Generis Database Rights means rights other than copyright resulting from Directive 96/9/EC of the European Parliament and of the Council of 11 March 1996 on the legal protection of databases, as amended and/or succeeded, as well as other essentially equivalent rights anywhere in the world.
- You means the individual or entity exercising the Licensed Rights under this Public License. Your has a corresponding meaning.