Antibacterial Activity Test of Ethanol Extract of Bitter Gourd Leaves (Momordica Charantia L) Against Acne-Causing Bacteria (Staphylococcus Epidermidis)
Keywords:
Antibacterial, Bitter Melon Leaves, Staphylococcus EpidermidisAbstract
Bitter melon leaves contain flavonoids, saponins, tannins and terpenoids, which have antibacterial potential. The aim of this research was to determine the antibacterial activity of an ethanol extract of bitter melon leaves. This antibacterial activity test was carried out using the disc diffusion method. The concentrations of bitter melon leaf ethanol extract used were 50%, 60%, and 70%. Clindamycin was used as a positive control and distilled water as a negative control. The data was obtained by using the one-way Anova statistical test. The test for antibacterial activity showed that the inhibitory zone was 9.07 mm wide at a 50% extract concentration, 10.72 mm wide at a 60% concentration, and 12.08 mm wide at a 70% concentration. The Sig value indicated that there was a significant difference between the positive control with extract concentrations of 50%, 60%, and 70%. 0.005. The ethanol extract of bitter melon has an inhibitory effect on the growth of Staphylococcus epidermidis bacteria.
References
Azizah, Z., & Wati, SW (2018). Phytochemical screening and determination of total flavonoid content of ethanol extract of bitter melon leaves (Momordica charantia L.). Higea Pharmaceutical Journal, 10(2), 163-172.
Harborne, J., 1987. Phytochemical Methods: A Guide to Modern Ways of Analyzing Plants. Bandung: ITB publisher
Hariadi, P., Azim, M., Yuliana, TP, & Febriani, Y. (2022). Gel Formulation of Renggak Fruit Peel Extract (Amomum Dealbatum Roxb) as an Anti-Acne Preparation. Medical Science: Pharmaceutical Scientific Journal, 7(1), 107-112.
Harith, SS, Mazlun, MH, Mydin, MM, Nawi, L., & Saat, R. (2018). Studies on phytochemical constituents and antimicrobial properties of Citrullus lanatus peels. Malaysian Journal of Analytical Sciences, 22(1), 151-156.
Lestari, RT, Gifanda, LZ, Kurniasari, EL, Harwiningrum, RP, Kelana, API, Fauziyah, K. & Priyandani, Y. (2021). Student Behavior Regarding How to Treat Acne. Journal of Community Pharmacy, 8(1), 15-19.
Leviyanti, M., & Sukamawati, S. (2021). Antibacterial Activity Test of Dragon Fruit Stem Extract (Hylocereus polyrhizus (FAC Weber) Britton & Rose) Against Staphylococcus aureus. Herbapharm: Journal of Herbs and Pharmacology, 3(1), 42-47.
Riferty, F. (2018). Antibacterial Activity Test of Bitter Gourd Seed Extracts and Fractions (Momordica Charantia L.) Against Propionibacterium Acnes Bacteria. Farmasyifa Pharmaceutical Scientific Journal, 1(2), 119-125.
Reusser, F. 1975. "Effect of lincomycin and clindamycin on peptide chain initiation". Antimicrob Agents Chemother. Vol. 7(1): pp. 32-37.
Sapitri, A., Nazara, PJ, & Asfianti, V. (2020). Test of the Effectiveness of Ethanol Extract of Jatropha Curcas (Jatropha curcas L.) Leaves Against Staphylococcus Epidermidis and Propionibacterium Acnes Bacteria in Vitro. Herbal Medicine Journal, 3(2), 10-18.
Sari, ZAA, & Febriawan, R. (2021). Differences in the Antibacterial Activity Test Results of the Well Diffusion and Kirby Bauer Methods on Bacterial Growth. Hutama Medika Journal, 2(04).
Seftiana, Rinda. 2017. Comparison of the antibacterial activity of water and methanol fractions of Srikaya (Annona Squamosa L.) leaves fermented with lactic acid (Lactobacillus bulgaricus) against Staphylococcus aureus. Thesis. Faculty of Pharmacy, Bhakty Wiyata Institute of Health Sciences, Kediri.
Soleh, Achmad Zanbar. 2005. Theoretical and Applicative Approaches to Statistical Science. Bandung: Science Engineering
Yani, ISY, Muthmainah, N., & Yasmina, A. (2020). Comparison of the Antibacterial Activity of Tanjung Leaf and Guava Leaf Extracts against Staphylococcus Aureus in Vitro. Homeostasis, 3(2), 277-2
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